IB ChemistryStructure 33.23.2.8
3.2.8HL

Mass Spectrometry

Finding molecular mass and structural fragments from m/z peaks.

🟣 This is Higher Level (HL) content.

Reading a Mass Spectrum

Peak What It Tells You
Molecular ion (M⁺) Highest m/z peak (ignoring isotope peaks) → gives the relative molecular mass
Base peak Most abundant fragment (tallest peak, set to 100%)
Fragment peaks Differences between M⁺ and fragments reveal structural groups

Common Fragment Losses

m/z Loss Fragment Lost
15 CH₃ (methyl)
17 OH (hydroxyl)
18 H₂O
28 CO or C₂H₄
29 CHO (aldehyde) or C₂H₅
45 OC₂H₅ or COOH
← 3.2.7 Stereoisomerism3.2.9 IR Spectroscopy (HL) →